In Vitro Mutagenesis pp 491-495

Part of the Methods in Molecular Biology book series (MIMB, volume 1498) | Cite as

Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent

Protocol

Abstract

Heavy water is a form of water that contains a heavier isotope of hydrogen (2H, also known as deuterium, D) or oxygen (17O or 18O). When using heavy water as a solvent, error-prone polymerase chain reaction (epPCR) can induce random mutations independent of the polymerase used or the composition of the PCR reaction mixture. This relatively new method can easily be combined with the existing epPCR methods to increase the rate of mutations.

Key words

Random mutagenesis Error-prone polymerase chain reaction (epPCR) Heavy water D2H218Stable isotope 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Graduate School of Human Development and EnvironmentKobe UniversityKobeJapan

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