Abstract
Genome-wide association (GWA) mapping is a powerful method for the identification of alleles that underlie quantitative traits. It enables one to understand how genetic variation translates into phenotypic variation. In particular, plant hormone signaling pathways play a key role in shaping phenotypes. This chapter presents a protocol for genome-wide association mapping of root traits of Arabidopsis thaliana in the context of hormone research. We describe a specific protocol for acquiring primary and lateral root trait data that is appropriate for GWA studies using FIJI (ImageJ), and subsequent GWA mapping using a user-friendly Internet application.
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Acknowledgement
We are grateful to Elke Barbez, Takehiko Ogura, and Santosh Satbhai for comments and suggestion for this protocol, Ãœmit Seren for information about the GWA-Portal, and Matthew Watson for editing.
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Ristova, D., Busch, W. (2017). Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research. In: Kleine-Vehn, J., Sauer, M. (eds) Plant Hormones. Methods in Molecular Biology, vol 1497. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6469-7_6
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DOI: https://doi.org/10.1007/978-1-4939-6469-7_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6467-3
Online ISBN: 978-1-4939-6469-7
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