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Imaging Golgi Outposts in Fixed and Living Neurons

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The Golgi Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1496))

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Abstract

Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize GOPs in living neurons.

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Acknowledgements

Research at the Laboratory of Neurobiology at INIMEC-CONICET-UNC is supported by grants from FONCyT, CONICET to A.C. M.B. is supported by a return home grant from ISN and FONCyT grant (PICT D 2013-1525). A.C. and M.B. are staff scientists from CONICET.

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Correspondence to Alfredo Caceres .

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Bisbal, M., Quassollo, G., Caceres, A. (2016). Imaging Golgi Outposts in Fixed and Living Neurons. In: Brown, W. (eds) The Golgi Complex. Methods in Molecular Biology, vol 1496. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6463-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6463-5_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6461-1

  • Online ISBN: 978-1-4939-6463-5

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