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DNA Nanostructure as Smart Carriers for Drug Delivery

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3D DNA Nanostructure

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1500))

Abstract

Self-assembled DNA nanostructures have recently emerged as a type of drug delivery carriers due to their suitable sizes, well-defined nanoscale shapes, precise spatial addressability, and excellent biocompatibility. Here, we describe practical procedures in detail for the design and construction of DNA nanostructures with different width and patterns by long rolling circle amplification (RCA) strands and a few short staples, and provide practical guidance and troubleshooting advice for delivering CpG immunostimulatory drugs with these RCA based DNA nanostructures.

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Acknowledgements

We appreciate financial support from the Ministry of Science and Technology of China (2012CB932600) and the National Science Foundation of China (91127037, 91123037, and 90913014).

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Authors and Affiliations

  1. Laboratory of Physical Biology, Chinese Academy of Sciences, Shanghai Institute of Applied Physics, Shanghai, P.O. Box 800-204, 201800, China

    Xiangyuan Ouyang, Jie Chao & Chunhai Fan

  2. Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi’an, 710127, People’s Republic of China

    Xiangyuan Ouyang

  3. Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu, 210023, China

    Jie Chao & Shao Su

Authors
  1. Xiangyuan Ouyang
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  2. Jie Chao
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  3. Shao Su
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  4. Chunhai Fan
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Corresponding authors

Correspondence to Jie Chao or Chunhai Fan .

Editor information

Editors and Affiliations

  1. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Yonggang Ke

  2. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Pengfei Wang

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Ouyang, X., Chao, J., Su, S., Fan, C. (2017). DNA Nanostructure as Smart Carriers for Drug Delivery. In: Ke, Y., Wang, P. (eds) 3D DNA Nanostructure. Methods in Molecular Biology, vol 1500. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6454-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6454-3_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6452-9

  • Online ISBN: 978-1-4939-6454-3

  • eBook Packages: Springer Protocols

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