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Genotyping DNA Variants with High-Resolution Melting Analysis

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Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1492))

Abstract

High-resolution melting analysis (HRMA) is a simple, quick, and effective method to scan and screen PCR amplicons for sequence variants. HRMA is a nondestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.

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Correspondence to Rolf H. A. M. Vossen .

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Vossen, R.H.A.M. (2017). Genotyping DNA Variants with High-Resolution Melting Analysis. In: White, S., Cantsilieris, S. (eds) Genotyping. Methods in Molecular Biology, vol 1492. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6442-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6442-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6440-6

  • Online ISBN: 978-1-4939-6442-0

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