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Cleavable Linkers in Chemical Proteomics Applications

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Activity-Based Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1491))

Abstract

The discovery of the protein targets of small molecule probes is a crucial aspect of activity-based protein profiling and chemical biology. Mass spectrometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their different chemistries, and describe how they aid in protein identification.

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Acknowledgements

We acknowledge funding by the Chinese Scholarship Council (to Y.Y.), the Slovenian Research Agency (ARRS grant to M.F.), the Deutsche Forschungsgemeinschaft (to S.V.) and the Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein- Westfalen.

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Authors and Affiliations

  1. Lehrstuhl für Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany

    Yinliang Yang

  2. Department of Biochemistry, Molecular and Structural Biology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana, Slovenia

    Marko Fonović

  3. Department of Cellular and Molecular Medicine, KU Leuven - University of Leuven, Herestr. 49 box 802, 3000 Leuven, Belgium, 3000, Leuven, Belgium

    Steven H. L. Verhelst

  4. Leibniz Institute for AnalyticalSciences ISAS, Otto-Hahn-Str. 6b, 44227, Dortmund, Germany

    Steven H. L. Verhelst

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  1. Yinliang Yang
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  2. Marko Fonović
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  3. Steven H. L. Verhelst
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Correspondence to Steven H. L. Verhelst .

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Editors and Affiliations

  1. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Herman S. Overkleeft

  2. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Bogdan I. Florea

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Yang, Y., Fonović, M., Verhelst, S.H.L. (2017). Cleavable Linkers in Chemical Proteomics Applications. In: Overkleeft, H., Florea, B. (eds) Activity-Based Proteomics. Methods in Molecular Biology, vol 1491. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6439-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6439-0_14

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