Abstract
Recombinant proteins are important tools for understanding molecular functions in vitro. Recent progress in the generation of recombinant proteins is amazing. However, when we plan to produce them, we should choose the best method according to the nature and the use of the target recombinant protein. Degradation and mis-folding are major problems in producing active recombinant CCN2. The method shown in this chapter describes the appropriate conditions under which we can produce CCN2 and its truncated fragments in Escherichia coli.
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Acknowledgement
We thank Yoshiko Miyake for her secretarial assistance. This work was supported by the programs JSPS KAKENHI Grants-in-Aid for Scientific Research (C), No. JP15 K1103807 (to EA) and (B) No. JP15H05014 (to MT).
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Aoyama, E., Hattori, T., Kubota, S., Takigawa, M. (2017). Production of Recombinant CCN2 Protein in Escherichia coli . In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_8
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DOI: https://doi.org/10.1007/978-1-4939-6430-7_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6428-4
Online ISBN: 978-1-4939-6430-7
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