CCN Proteins pp 487-493 | Cite as
CCN Detection of Cancer Tissues by Immunohistochemistry Staining
Protocol
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Abstract
CCN family members are involved in many physiologic and pathological functions, and be detected in many different cancer types. Immunohistochemistry (IHC) is an important diagnostic pathology tool to demonstrate protein expression in clinical and research fields. Here, we explain the preparation of sample slides, staining procedure, and the problems that might be met during the time. The differential staining of CCN proteins is shown in breast cancer, oral cancer, lung cancer, colorectal cancer, and gastric cancer.
Key words
CCN Immunohistochemistry Bovine serum albumin Antigen retrieval Polymer-horseradish peroxidaseNotes
Acknowledgement
This work was supported by Ministry of Science and Technology, R.O.C, and National Taiwan University.
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