Abstract
Transcytosis is a mechanism for the transcellular transport of biomolecules. Analysis of transcytosis is frequently performed in cells with distinct polarity, such as brain endothelial cells. However, in cells without evident polarity, analysis of transcytosis has not been performed. Here, we describe a method for analyzing transcytosis of a CCN family protein through chondrocytic cells having no apparent polarity.
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Acknowledgement
This work was supported by the programs Grants-in-Aid for Scientific Research (S) [#JP19109008 to M.T.] and (B) [#JP15H05014 to M.T.] and (C) [#JP25462886 to S.K.] and Grants-in-Aid for Exploratory Research (to M.T.) from the Japan Society for the Promotion of Science; and by a grant from the Ryobi Teien Memory Foundation (to K.K.).
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Kawata, K., Kubota, S., Takigawa, M. (2017). Analysis of Transcytosis of CCN2 by Chondrocytes. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_33
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DOI: https://doi.org/10.1007/978-1-4939-6430-7_33
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6428-4
Online ISBN: 978-1-4939-6430-7
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