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Expression of CCN Genes and Proteins in Human Skin: Methods and Protocols

Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 1489)

Abstract

CCN proteins are vital to a variety of biological processes that contribute to the normal development and function of skin. Quantitative real-time reverse transcriptase PCR (qPCR) quantifies mRNA levels of these key genes. Immunostaining localizes CCN proteins within skin tissue, and provides semiquantitative information regarding relative levels of the proteins. Reliable determinations of the expression levels of CCN genes and proteins are essential to uncovering their roles in skin physiology and pathology.

Key words

CCN proteins Gene expression Real-time q-PCR Immunostaining Skin 
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Notes

Acknowledgement

This work was supported by grants AG019364, ES014697, and AG031452, from the National Institutes of Health, Bethesda, MD.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of DermatologyUniversity of Michigan Medical SchoolAnn ArborUSA

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