Abstract
Recent progress in molecular imaging technology has had a strong impact on improving our understanding of molecular translocation, receptor internalization, and interactions in living cells. The protocol in this chapter introduces an optimized technique for intracellular localization of CCN2 and CCN3 in live cells, one using GFP-tagged CCN2 and Halo-tagged CCN3.
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Acknowledgements
We thank Drs. Satoshi Kubota and Takashi Nishida for useful suggestions, and Ms. Yoshiko Miyake and Ayako Ogo for technical and secretarial assistance. This work was supported by grants from the program Grants-in-aid for Scientific Research (B) [#JP24390415] and (B) [#JP15H05014] to M.T. and (C) [#JP254628889] to T.H. and by the Foundation for Growth Science to T.H.
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Hattori, T., Hoshijima, M., Takigawa, M. (2017). Protein Imaging of CCN2 and CCN3 in Living Cells. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_20
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DOI: https://doi.org/10.1007/978-1-4939-6430-7_20
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