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CCN Proteins pp 127-138 | Cite as

ELISA of CCN Family Proteins in Body Fluids Including Serum and Plasma

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Part of the Methods in Molecular Biology book series (MIMB, volume 1489)

Abstract

Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.

Key words

CCN family ELISA Platelet Fibrosis 
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Notes

Acknowledgement

We gratefully thank Dr. Tarek Abd El Kader for critically reading the manuscript.

Funding

This study was supported by grants from the program Grants-in-aid for Scientific Research (B) [JP24390415] and (B) [JP15H05014] to M.T. and (C) [JP25462886] to S.K. from the Japan Society for the Promotion of Science and from Wesco Scientific Promotion Foundation.

Conflict of Interest: The authors have no conflict of interest to declare.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Advanced Research Center for Oral and Craniofacial SciencesOkayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayamaJapan
  2. 2.Department of MembraneBiochemistryOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayamaJapan
  3. 3.Advanced Research Center for Oral and Craniofacial SciencesOkayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayamaJapan
  4. 4.Department of Oral Biochemistry, Division of Oral Structure, Function, and DevelopmentAsahi University School of DentistryGifuJapan

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