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ERK Signaling pp 367-378 | Cite as

Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis

  • Takaaki MatsuiEmail author
  • Yasumasa Bessho
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1487)

Abstract

During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.

Key words

MAPK FGF Somite Zebrafish Immunohistochemistry Quantitative analysis 

Notes

Acknowledgement

We thank Fiqri Dizar Khaidizar and Ryutaro Akiyama for critical reading of the manuscript. We are grateful to the Ministry of Education, Culture, Sports, Science and Technology (MEXT), the Uehara Memorial Foundation, the Nakajima Foundation and the Mochida Memorial Foundation for past and current support.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Gene Regulation ResearchNara Institute of Science and TechnologyTakayamaJapan

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