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Analyzing Synthetic Promoters Using Arabidopsis Protoplasts

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Plant Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1482))

Abstract

This chapter describes a transient protoplast co-transfection method that can be used to quantitatively study in vivo the activity and function of promoters and promoter elements (reporters), and their induction or repression by transcription factors (effectors), stresses, hormones, or metabolites. A detailed protocol for carrying out transient co-transfection assays with Arabidopsis At7 protoplasts and calculating the promoter activity is provided.

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Author information

Authors and Affiliations

  1. Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany

    Ralf Stracke, Katharina Thiedig, Melanie Kuhlmann & Bernd Weisshaar

Authors
  1. Ralf Stracke
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  2. Katharina Thiedig
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  3. Melanie Kuhlmann
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  4. Bernd Weisshaar
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Corresponding author

Correspondence to Bernd Weisshaar .

Editor information

Editors and Affiliations

  1. Institut für Genetik, Technische Universität Braunschweig, Braunschweig, Germany

    Reinhard Hehl

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Stracke, R., Thiedig, K., Kuhlmann, M., Weisshaar, B. (2016). Analyzing Synthetic Promoters Using Arabidopsis Protoplasts. In: Hehl, R. (eds) Plant Synthetic Promoters. Methods in Molecular Biology, vol 1482. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6396-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6396-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6394-2

  • Online ISBN: 978-1-4939-6396-6

  • eBook Packages: Springer Protocols

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