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Wnt-Dependent Control of Cell Polarity in Cultured Cells

  • Kristin B. Runkle
  • Eric S. WitzeEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1481)

Abstract

The secreted ligand Wnt5a regulates cell polarity and polarized cell movement during development by signaling through the poorly defined noncanonical Wnt pathway. Cell polarity regulates most aspects of cell behavior including the organization of apical/basolateral membrane domains of epithelial cells, polarized cell divisions along a directional plane, and front rear polarity during cell migration. These characteristics of cell polarity allow coordinated cell movements required for tissue formation and organogenesis during embryonic development. Genetic model organisms have been used to identify multiple signaling pathways including Wnt5a that are required to establish cell polarity and regulate polarized cell behavior. However, the downstream signaling events that regulate these complex cellular processes are still poorly understood. The methods below describe assays to study Wnt5a-induced cell polarity in cultured cells, which may facilitate our understanding of these complex signaling pathways.

Key words

Wnt5a Noncanonical Wnt Cell polarity Cell adhesion molecules Cultured cells 

References

  1. 1.
    Wallingford JB, Vogeli KM, Harland RM (2001) Regulation of convergent extension in Xenopus by Wnt5a and Frizzled-8 is independent of the canonical Wnt pathway. Int J Dev Biol 45:225–227PubMedGoogle Scholar
  2. 2.
    Yamaguchi TP, Bradley A, McMahon AP, Jones S (1999) A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo. Development 126:1211–1223PubMedGoogle Scholar
  3. 3.
    Wyngaarden LA, Vogeli KM, Ciruna BG, Wells M, Hadjantonakis AK, Hopyan S (2010) Oriented cell motility and division underlie early limb bud morphogenesis. Development 137:2551–2558CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Weeraratna AT, Jiang Y, Hostetter G, Rosenblatt K, Duray P, Bittner M, Trent JM (2002) Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma. Cancer Cell 1:279–288CrossRefPubMedGoogle Scholar
  5. 5.
    Wang W, Snyder N, Worth AJ, Blair IA, Witze ES (2015) Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production. Oncogenesis 4:e154CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Witze ES, Litman ES, Argast GM, Moon RT, Ahn NG (2008) Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors. Science 320:365–369CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Wang W, Runkle KB, Terkowski SM, Ekaireb RI, Witze ES (2015) Protein depalmitoylation is induced by Wnt5a and promotes polarized cell behavior. J Biol Chem 290:15707–15716CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Zicha D, Dunn GA, Brown AF (1991) A new direct-viewing chemotaxis chamber. J Cell Sci 99:769–775PubMedGoogle Scholar
  9. 9.
    Willert K, Brown JD, Danenberg E, Duncan AW, Weissman IL, Reya T, Yates JR III, Nusse R (2003) Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423:448–452CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Cancer BiologyUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA
  3. 3.Abramson Family Cancer Research InstituteUniversity of PennsylvaniaPhiladelphiaUSA

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