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Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway

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Wnt Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1481))

Abstract

Wnt pathways are critical for embryonic development and adult tissue homeostasis in all multicellular animals. Many regulatory mechanisms exist to control proper signaling output. Recent studies suggest that cell surface Wnt receptor level is controlled by ubiquitination, and serve as a critical regulatory point of Wnt pathway activity as it determines the responsiveness of cells to Wnt signal. Here, we describe flow cytometry, cell surface protein biotinylation, and immunofluorescence pulse-chase methods to probe the surface expression, ubiquitination, and internalization of the Wnt receptors FZD and LRP6.

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Author information

Authors and Affiliations

  1. Immuno-oncology, Novartis Institutes for Biomedical Research, Cambridge, MA, USA

    Xiaomo Jiang

  2. Developmental and Molecular Pathways, Novartis Institutes for Biomedical Research, 250 Massachusetts Avenue, Cambridge, MA, 02139, USA

    Feng Cong

Authors
  1. Xiaomo Jiang
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  2. Feng Cong
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Corresponding author

Correspondence to Feng Cong .

Editor information

Editors and Affiliations

  1. University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Quinn Barrett

  2. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Lawrence Lum

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Jiang, X., Cong, F. (2016). Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway. In: Barrett, Q., Lum, L. (eds) Wnt Signaling. Methods in Molecular Biology, vol 1481. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6393-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6393-5_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6391-1

  • Online ISBN: 978-1-4939-6393-5

  • eBook Packages: Springer Protocols

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