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Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo

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Wnt Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1481))

Abstract

The activity of tankyrase (Tnks) enzymes modulates the activity of the β-catenin destruction complex in the Wnt/β-catenin signaling pathway. Here, we describe a method for determining the accessibility of various zebrafish tissues in vivo and in vitro to small molecule inhibitors of Tankyrase enzymes. This biochemical assay will facilitate chemically based studies focused on understanding the role of Tankyrase in cell fate reprogramming and tissue homeostasis and provide insights into the potential role of Wnt/β-catenin signaling in these processes.

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Author information

Authors and Affiliations

  1. Pediatrics and Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Jesung Moon

  2. Pediatrics, Molecular Biology and Internal Medicine, University of Texas Southwestern Medical Center, ND3.120C, 5323 Harry Hines Blvd., Dallas, TX, 75390-8543, USA

    James F. Amatruda

Authors
  1. Jesung Moon
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  2. James F. Amatruda
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Corresponding author

Correspondence to James F. Amatruda .

Editor information

Editors and Affiliations

  1. University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Quinn Barrett

  2. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Lawrence Lum

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Moon, J., Amatruda, J.F. (2016). Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo. In: Barrett, Q., Lum, L. (eds) Wnt Signaling. Methods in Molecular Biology, vol 1481. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6393-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6393-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6391-1

  • Online ISBN: 978-1-4939-6393-5

  • eBook Packages: Springer Protocols

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