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Visualizing Wnt Palmitoylation in Single Cells

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Wnt Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1481))

Abstract

Wnt palmitoylation regulates its secretion and signaling activity in cells. Methods to monitor cellular Wnt palmitoylation are instrumental in investigating Wnt activity, secretion, and its interaction with cellular membrane compartments. This protocol describes a method we have recently developed to detect cellular Wnt palmitoylation. The method, combining click chemistry, bio-orthogonal fatty acid probes, and proximity ligation assay (PLA), provides high sensitivity and subcellular resolution for detection of Wnt palmitoylation. It is also compatible with multiple imaging platforms, and is applicable to detecting palmitoylated forms of other fatty acylated proteins.

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Acknowledgment

We acknowledge use of microscopes at the Center for Advanced Light Microscopy (CALM) at Genentech.

Author information

Authors and Affiliations

  1. Department of Early Discovery Biochemistry, Genentech, South San Francisco, CA, USA

    Xinxin Gao & Rami N. Hannoush

Authors
  1. Xinxin Gao
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  2. Rami N. Hannoush
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Corresponding author

Correspondence to Rami N. Hannoush .

Editor information

Editors and Affiliations

  1. University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Quinn Barrett

  2. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Lawrence Lum

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Gao, X., Hannoush, R.N. (2016). Visualizing Wnt Palmitoylation in Single Cells. In: Barrett, Q., Lum, L. (eds) Wnt Signaling. Methods in Molecular Biology, vol 1481. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6393-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6393-5_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6391-1

  • Online ISBN: 978-1-4939-6393-5

  • eBook Packages: Springer Protocols

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