Abstract
Polycomb complexes are essential regulators of embryonic and adult stem cells, highly conserved from flies to mammals. Traditionally, their study was based on biochemical and genetic approaches. More recently, the development of novel technologies and the improvement and standardization of existing ones has allowed to address previously unexplored aspects of Polycomb biology, such as dynamics and regulation. In this chapter, relevant researchers in the field discuss novel technologies aimed at dissecting the dynamics of Polycomb complexes in normal and pathological conditions.
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References
Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276(5688):565–570
Sato T, Denell RE (1985) Homoeosis in Drosophila: anterior and posterior transformations of Polycomb lethal embryos. Dev Biol 110(1):53–64
Hobert O, Jallal B, Ullrich A (1996) Interaction of Vav with ENX-1, a putative transcriptional regulator of homeobox gene expression. Mol Cell Biol 16(6):3066–3073
Schumacher A, Faust C, Magnuson T (1996) Positional cloning of a global regulator of anterior-posterior patterning in mice. Nature 384(6610):648
Haupt Y et al (1991) Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice. Cell 65(5):753–763
van Lohuizen M et al (1991) Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging. Cell 65(5):737–752
Bracken AP et al (2006) Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions. Genes Dev 20(9):1123–1136
Lanzuolo C, Orlando V (2012) Memories from the polycomb group proteins. Annu Rev Genet 46:561–589
Cesarini E et al (2015) Lamin A/C sustains PcG protein architecture, maintaining transcriptional repression at target genes. J Cell Biol 211(3):533–551
Strutt H, Paro R (1997) The polycomb group protein complex of Drosophila melanogaster has different compositions at different target genes. Mol Cell Biol 17(12):6773–6783
Franke A et al (1992) Polycomb and polyhomeotic are constituents of a multimeric protein complex in chromatin of Drosophila melanogaster. EMBO J 11(8):2941–2950
Morey L et al (2015) Polycomb regulates mesoderm cell fate-specification in embryonic stem cells through activation and repression mechanisms. Cell Stem Cell 17(3):300–315
Misteli T (2007) Beyond the sequence: cellular organization of genome function. Cell 128(4):787–800
Delest A, Sexton T, Cavalli G (2012) Polycomb: a paradigm for genome organization from one to three dimensions. Curr Opin Cell Biol 24(3):405–414
Chan TF, Vese LA (2001) Active contours without edges. IEEE Trans Image Process 10(2):266–277
El-Zaart A (2010) Images thresholding using ISODATA technique with gamma distribution. Pattern Recogn Image Anal 20(1):20–41
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Palacios, D. (2016). The Dynamics of Polycomb Complexes. In: Lanzuolo, C., Bodega, B. (eds) Polycomb Group Proteins. Methods in Molecular Biology, vol 1480. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6380-5_12
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DOI: https://doi.org/10.1007/978-1-4939-6380-5_12
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