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Use of Flow Focusing Technique for Microencapsulation of Myoblasts

Part of the Methods in Molecular Biology book series (MIMB,volume 1479)


Alginate cell microencapsulation implies the immobilization of cells within a polymeric membrane that allows the bidirectional diffusion of nutrients and oxygen inside the microcapsules and the release of waste and therapeutic molecules outside them. This technology has been applied to several cell types and it has been extensively described with pancreatic islets. However, other cells such as myoblasts are being currently studied and showing high interest. Moreover, different systems and approaches have been developed for cell encapsulation such as electrostatic extrusion and Flow focusing technology. When Flow focusing technology is applied for myoblast encapsulation, several factors should be considered, such as the pressure, the flow of the system, or the diameter size of the nebulizer, which will determine the final diameter size and shape of the microcapsules containing the myoblasts. Finally, viability of encapsulated myoblasts needs to be assessed before further studies are performed.

Key words

  • Flow focusing
  • Alginate
  • Cell microencapsulation
  • Myoblasts
  • Erythropoietin
  • Drug delivery
  • Nanomedicine

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Correspondence to J. L. Pedraz Ph.D. .

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Ciriza, J., Saenz del Burgo, L., Hernández, R.M., Orive, G., Pedraz, J.L. (2017). Use of Flow Focusing Technique for Microencapsulation of Myoblasts. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY.

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6362-1

  • Online ISBN: 978-1-4939-6364-5

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