Abstract
Colloidal gold probes, including protein A/G/L, IgG, F(ab′)2, and streptavidin labeled with gold particles, are useful tools to localize antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.
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References
Roth J (1982) The protein A-gold (pAg) technique—a qualitative and quantitative approach for antigen localization on thin sections. In: Bullock GR, Petrusz P (eds) Techniques in immunocytochemistry, vol 1. Academic, London, pp 108–133
Tokuyasu KT (1980) Immunochemistry on ultrathin frozen sections. Histochem J 12:381–403
Frens G (1973) Controlled nucleation for the regulation of particle size in monodisperse gold suspensions. Nature Phys Sci 241:20–22
Baschong W, Lucocq JM, Roth J (1985) “Thiocyanate gold”: small (2–3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy. Histochemistry 83:409–411
Tschopp J, Podack ER, Müller-Eberhard HJ (1982) Ultrastructure of the membrane attack complex of complement: detection of the tetramolecular C9-polymerizing complex C5b-8. Proc Natl Acad Sci U S A 79:7474–7478
De Roe C, Courtoy PJ, Baudhuin P (1987) A model of protein-colloidal gold interaction. J Histochem Cytochem 35:1191–1198
De Mey J, Moeremans M, Geuens G, Nuydens R, De Brabander M (1981) High resolution light and electron microscopic localization of tubulin with the IGS (Immuno Gold Staining) method. Cell Biol Int Rep 5:889–899
Ackerman GA, Wolken KW, Gelder FB (1980) Surface distribution of monosialoganglioside Gm1 on human blood cells and the effect of exogenous GM1 and neuraminidase on cholera toxin surface labeling. A quantitative immunocytochemical study. J Histochem Cytochem 28:1100–1112
Bonnard C, Papermaster DS, Kraehenbuhl J-P (1984) The streptavidin-biotin bridge technique: application in light and electron microscope immunocytochemistry. In: Polak JM, Varndell IM (eds) Immunolabeling for electron microscopy. Elsevier, Amsterdam, pp 95–111
Yokota S (1988) Effect of particle size on labeling density for catalase in protein A-gold immunocytochemistry. J Histochem Cytochem 36:107–109
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Yokota, S. (2016). Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab′)2, and Streptavidin. In: Schwartzbach, S., Skalli, O., Schikorski, T. (eds) High-Resolution Imaging of Cellular Proteins. Methods in Molecular Biology, vol 1474. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6352-2_4
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DOI: https://doi.org/10.1007/978-1-4939-6352-2_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6350-8
Online ISBN: 978-1-4939-6352-2
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