Meiosis pp 157-174 | Cite as

Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast

  • Amy J. MacQueenEmail author
  • Beth Rockmill
Part of the Methods in Molecular Biology book series (MIMB, volume 1471)


The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast, S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments.

Key words

Meiosis Chromatin Synaptonemal complex Conditional expression Yeast 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Molecular Biology and BiochemistryWesleyan UniversityMiddletownUSA
  2. 2.Howard Hughes Medical Institute and Department of Molecular and Cell BiologyUniversity of California-BerkeleyBerkeleyUSA

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