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Meiosis pp 133-145 | Cite as

Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora

  • Frederick J. Bowring
  • P. Jane Yeadon
  • David E. A. CatchesideEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1471)

Abstract

We have built a series of Neurospora crassa strains containing alleles of green fluorescent protein (GFP) to provide a visual phenotype for investigating meiotic recombination. These strains provide a convenient means of screening the Neurospora knockout library for genes involved in genetic recombination. They permit rapid analysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for GFP. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.

Key words

Neurospora Meiosis Recombination Tetrad analysis Octads Crossing over GFP 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Frederick J. Bowring
    • 1
  • P. Jane Yeadon
    • 1
  • David E. A. Catcheside
    • 1
    Email author
  1. 1.School of Biological SciencesFlinders UniversityAdelaideAustralia

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