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Meiosis pp 99-122 | Cite as

Ribosome Profiling for the Analysis of Translation During Yeast Meiosis

  • Caia Duncan
  • Juan MataEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1471)

Abstract

Ribosome profiling provides a genome-wide view of translation with unprecedented resolution. Application of this approach to fission and budding yeast revealed widespread regulation of translational efficiency, translation of short open reading frames on unannotated transcripts, and frequent translation of open reading frames in 5′ leader sequences. We present here a detailed protocol for the application of ribosome profiling to meiotic fission yeast cells, although the approach should be easily adapted to budding yeast.

Key words

S. pombe Meiosis Translation Protein synthesis Ribo-seq Ribosome profiling NGS 

Notes

Acknowledgement

This was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) project grant to Juan Mata (BB/M021483/1). We thank Maria J. Amorim for help with sucrose gradient centrifugation.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of CambridgeCambridgeUK

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