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Meiosis pp 289-303 | Cite as

Analysis of Chromatin Dynamics During Drosophila Spermatogenesis

  • Tim Hundertmark
  • Ina Theofel
  • Zeynep Eren-Ghiani
  • David Miller
  • Christina RathkeEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1471)

Abstract

In the course of spermatogenesis, germ cells undergo dramatic morphological changes that affect almost all cellular components. Therefore, it is impossible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating Drosophila male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat Drosophila sperm before immunofluorescence staining to help reveal epitopes in the highly condensed sperm chromatin that otherwise may be inaccessible to antibodies.

Key words

Drosophila Spermatogenesis Chromatin Immunofluorescence Sperm decondensation 

Notes

Acknowledgment

This work was supported by the German Research Foundation (DFG) within the TRR 81 and a research grant to CR (RA 2150/2-1). DM and ZE-G were supported by REPRO-TRAIN Marie Curie Initial Training Network (PITN-2011-289880).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Tim Hundertmark
    • 1
  • Ina Theofel
    • 1
  • Zeynep Eren-Ghiani
    • 1
  • David Miller
    • 2
  • Christina Rathke
    • 1
    Email author
  1. 1.Department of BiologyPhilipps-University MarburgMarburgGermany
  2. 2.Institute of Cardiovascular and Metabolic Medicine (LICAMM)University of LeedsWest YorkshireUK

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