Analysis of Chromatin Dynamics During Drosophila Spermatogenesis
In the course of spermatogenesis, germ cells undergo dramatic morphological changes that affect almost all cellular components. Therefore, it is impossible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating Drosophila male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat Drosophila sperm before immunofluorescence staining to help reveal epitopes in the highly condensed sperm chromatin that otherwise may be inaccessible to antibodies.
Key wordsDrosophila Spermatogenesis Chromatin Immunofluorescence Sperm decondensation
This work was supported by the German Research Foundation (DFG) within the TRR 81 and a research grant to CR (RA 2150/2-1). DM and ZE-G were supported by REPRO-TRAIN Marie Curie Initial Training Network (PITN-2011-289880).
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