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Meiosis pp 277-288 | Cite as

Drosophila Male Meiosis

  • Silvia Bonaccorsi
  • Maurizio GattiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1471)

Abstract

In Drosophila males, there is no synaptonemal complex and recombination does not occur. Thus, Drosophila male meiosis is a good model system for the analysis of achiasmate chromosome segregation. In addition, due to their large size, the meiotic spindles of Drosophila males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live Drosophila testes and for preparation of fixed meiotic chromosomes and spindles.

Key words

Male meiosis Live analysis Spermatogenesis Chromosomes Meiotic spindle Immunostaining Drosophila 

Notes

Acknowledgments

This work has been supported by a PRIN grant to SB and an AIRC grant (IG 16020) to MG.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Biology and Biotechnology “Charles Darwin”, SapienzaUniversity of RomeRomeItaly
  2. 2.Institute of Molecular Biology and Pathology (IBPM) of CNRRomeItaly

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