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High Throughput siRNA Screening Using Reverse Transfection

  • Carina von SchantzEmail author
  • Jani Saarela
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1470)

Abstract

RNA interference (RNAi) is a commonly used technique to knockdown gene function. Here, we describe a high throughput screening method for siRNA mediated gene silencing of the breast cancer cell line MDA-MB-231 using reverse transfection. Furthermore, we describe the setup for two separate methods for detecting viable and dead cells using either homogenous assays or image-based analysis.

Key words

RNAi Reverse transfection Gene silencing Acoustic droplet ejection siRNA screening High throughput screening 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute for Molecular Medicine Finland (FIMM)University of HelsinkiHelsinkiFinland

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