In Vitro High-Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development
High-throughput RNA interference (HT-RNAi) is a powerful tool that can be used to knock down gene expression in order to identify novel genes and pathways involved in many cellular processes. It is a systematic, yet unbiased, approach to identify essential or synthetic lethal genes that promote cell survival in diseased cells as well as genes that confer resistance or sensitivity to drug treatment. This information serves as a foundation for enhancing current treatments for cancer and other diseases by identifying new drug targets, uncovering potential combination therapies, and helping clinicians match patients with the most effective treatment based on genetic information. Here, we describe the method of performing an in vitro HT-RNAi screen using chemically synthesized siRNA.
Key wordsHigh-throughput RNA interference (RNAi) siRNA Target discovery Drug development Drug sensitizer Gene knockdown Functional genomics Gene silencing Essential gene Synthetic lethal
We would like to thank TGen for their support, Mr. Chris Sereduk for editing, and Springer Publishing.
- 7.Life Technologies. Duration of siRNA induced silencing: your questions answered. https://www.lifetechnologies.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/duration-of-sirna-induced-silencing.html. Accessed 30 Jul 2015