In Vitro High-Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development

  • Hongwei YinEmail author
  • Michelle Kassner
Part of the Methods in Molecular Biology book series (MIMB, volume 1470)


High-throughput RNA interference (HT-RNAi) is a powerful tool that can be used to knock down gene expression in order to identify novel genes and pathways involved in many cellular processes. It is a systematic, yet unbiased, approach to identify essential or synthetic lethal genes that promote cell survival in diseased cells as well as genes that confer resistance or sensitivity to drug treatment. This information serves as a foundation for enhancing current treatments for cancer and other diseases by identifying new drug targets, uncovering potential combination therapies, and helping clinicians match patients with the most effective treatment based on genetic information. Here, we describe the method of performing an in vitro HT-RNAi screen using chemically synthesized siRNA.

Key words

High-throughput RNA interference (RNAi) siRNA Target discovery Drug development Drug sensitizer Gene knockdown Functional genomics Gene silencing Essential gene Synthetic lethal 



We would like to thank TGen for their support, Mr. Chris Sereduk for editing, and Springer Publishing.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Cancer and Cell Biology Division (CCB)Translational Genomics Research Institute (TGen)PhoenixUSA

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