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High-Throughput RNAi Screening pp 121–135Cite as

Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1470))

Abstract

The intrinsic limitations of 2D monolayer cell culture models have prompted the development of 3D cell culture model systems for in vitro studies. Multicellular tumor spheroid (MCTS) models closely simulate the pathophysiological milieu of solid tumors and are providing new insights into tumor biology as well as differentiation, tissue organization, and homeostasis. They are straightforward to apply in high-throughput screens and there is a great need for the development of reliable and robust 3D spheroid-based assays for high-throughput RNAi screening for target identification and cell signaling studies highlighting their potential in cancer research and treatment. In this chapter we describe a stringent standard operating procedure for the use of MCTS for high-throughput RNAi screens.

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Author information

Authors and Affiliations

  1. Division of Cancer Medicine, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, 77054, USA

    LaKesla R. Iles & Geoffrey A. Bartholomeusz Ph.D.

Authors
  1. LaKesla R. Iles
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  2. Geoffrey A. Bartholomeusz Ph.D.
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Corresponding author

Correspondence to Geoffrey A. Bartholomeusz Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Molecular Medicine, Phoenix Children’s Hospital, Phoenix, Arizona, USA

    David O. Azorsa

  2. Constellation Pharmaceuticals, Cambridge, Massachusetts, USA

    Shilpi Arora

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Iles, L.R., Bartholomeusz, G.A. (2016). Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens. In: Azorsa, D., Arora, S. (eds) High-Throughput RNAi Screening. Methods in Molecular Biology, vol 1470. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6337-9_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6337-9_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6335-5

  • Online ISBN: 978-1-4939-6337-9

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