Abstract
Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756–1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Whereas the previous demonstration was conducted through polyethylene glycol to mediate uptake of DNA into tobacco protoplasts, we now describe protocols for using biolistic transformation to stack DNA in tobacco and rice.
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Acknowledgments
This work received support from Guangdong Province, China Talent Funds 2010 and MOST/Ministry of Agriculture Grant 2010ZX08010-001. Authors also affiliated with the Key laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement.
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Li, R., Han, Z., Hou, L., Kaur, G., Yin, Q., Ow, D.W. (2016). Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase. In: Murata, M. (eds) Chromosome and Genomic Engineering in Plants. Methods in Molecular Biology, vol 1469. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4931-1_2
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DOI: https://doi.org/10.1007/978-1-4939-4931-1_2
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