Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy
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The Drosophila ovary represents a key in vivo model used to study germline stem cell (GSC) maintenance and stem cell daughter differentiation because these cells and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the Drosophila ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.
Key wordsDrosophila ovary Live-cell imaging Confocal microscopy Molecular dynamics Cellular dynamics
We would like to thank P. Robin Hiesinger and Neset Ozel for their input and advice while developing this protocol.