Germline Stem Cells pp 49-62
Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells
Asymmetric cell division (ACD) is utilized in many stem cell systems to produce two daughter cells with different cell fates. Despite the fundamental importance of ACD during development and tissue homeostasis, the nature of ACD is far from being fully understood. Step-by-step observation of events during ACD allows us to understand processes that lead to ACD. Here we describe examples of how we evaluate ACD in vivo using the Drosophila male germline stem cell system.
Key wordsAsymmetric cell division (ACD) Drosophila male germline stem cells (GSCs) Centrosome orientation Spindle orientation Centrosome orientation checkpoint (COC)
- 21.Cheng J, Hunt AJ (2009) Time-lapse live imaging of stem cells in Drosophila testis. Current Protoc Stem Cell Biol Chapter 2: Unit 2E.2Google Scholar