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Capillary Electrophoresis of Proteins and Peptides pp 25–41Cite as

Capillary Zone Electrophoresis–Mass Spectrometry of Intact Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1466))

Abstract

Capillary electrophoresis (CE) coupled with mass spectrometry (MS) has proven to be a powerful analytical tool for the characterization of intact proteins. It combines the high separation efficiency, short analysis time, and versatility of CE with the mass selectivity and sensitivity offered by MS detection. This chapter focuses on important practical considerations when applying CE-MS for the analysis of intact proteins. Technological aspects with respect to the use of CE-MS interfaces and application of noncovalent capillary coatings preventing protein adsorption are treated. Critical factors for successful protein analysis are discussed and four typical CE-MS systems are described demonstrating the characterization of different types of intact proteins by CE-MS. These methodologies comprise the use of sheath-liquid and sheathless CE-MS interfaces, and various types of noncovalent capillary coatings allowing efficient and reproducible protein separations. The discussion includes the analysis of lysozyme-drug conjugates and the therapeutic proteins human growth hormone, human interferon-β-1a, and human erythropoietin.

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Author information

Authors and Affiliations

  1. Division of BioAnalytical Chemistry, VU University, de Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands

    Elena Domínguez-Vega, Rob Haselberg & Govert W. Somsen

Authors
  1. Elena Domínguez-Vega
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  2. Rob Haselberg
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  3. Govert W. Somsen
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Corresponding author

Correspondence to Govert W. Somsen .

Editor information

Editors and Affiliations

  1. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Nguyet Thuy Tran

  2. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Myriam Taverna

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Domínguez-Vega, E., Haselberg, R., Somsen, G.W. (2016). Capillary Zone Electrophoresis–Mass Spectrometry of Intact Proteins. In: Tran, N., Taverna, M. (eds) Capillary Electrophoresis of Proteins and Peptides. Methods in Molecular Biology, vol 1466. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4014-1_3

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  • DOI: https://doi.org/10.1007/978-1-4939-4014-1_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-4012-7

  • Online ISBN: 978-1-4939-4014-1

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