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Capillary Electrophoresis of Proteins and Peptides pp 1–10Cite as

Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)–Fluorescence Analysis of Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1466))

Abstract

Capillary electrophoresis (CE) coupled to fluorescence detection is an invaluable technique for the quantitative analysis of proteins of interest in the field of clinical diagnosis and quality control of novel biotechnology products. The various chemical and instrumental approaches that have been reported to carry out such sensitive analysis are described in this paper. To illustrate the contribution of CE to the analysis of therapeutic proteins, a detailed protocol for impurities profiling of a recombinant antibody sample using CE-LEDIF is given.

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Author information

Authors and Affiliations

  1. Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, 5 rue Jean Baptiste Clément, Châtenay-Malabry, 92290, France

    Isabelle Le Potier

  2. Picometrics Technologies, 478 rue de la Découverte, Labège, 31670, France

    Audrey Boutonnet

  3. IPBS, Université Paul Sabatier, Université de Toulouse, 205 Route de Narbonne, Toulouse, 31062, France

    Vincent Ecochard

  4. Laboratoire des IMRCP, Université Paul Sabatier, Université de Toulouse, 118 Route de Narbonne, Toulouse, 31062, France

    François Couderc

Authors
  1. Isabelle Le Potier
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  2. Audrey Boutonnet
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  3. Vincent Ecochard
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  4. François Couderc
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Corresponding author

Correspondence to François Couderc .

Editor information

Editors and Affiliations

  1. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Nguyet Thuy Tran

  2. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Myriam Taverna

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Le Potier, I., Boutonnet, A., Ecochard, V., Couderc, F. (2016). Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)–Fluorescence Analysis of Proteins. In: Tran, N., Taverna, M. (eds) Capillary Electrophoresis of Proteins and Peptides. Methods in Molecular Biology, vol 1466. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4014-1_1

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  • DOI: https://doi.org/10.1007/978-1-4939-4014-1_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-4012-7

  • Online ISBN: 978-1-4939-4014-1

  • eBook Packages: Springer Protocols

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