Abstract
Integrative analysis of microRNA (miRNA) and messenger RNA (mRNA) in Chronic Myeloid leukemia (CML) stem cells is an important technique to study the involvement of miRNA and their targets in CML stem cells self-renewal, maintenance, and therapeutic resistance. Here, we describe a simplified integrative analysis using Ingenuity Pathway Analysis software after performing proper RNA extraction, miRNA and mRNA microarray and data analysis.
The original version of this book was revised. An erratum to the book can be found at DOI: 10.1007/978-1- 4939-4011-0_20
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-4011-0_20
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Acknowledgments
The authors thank the Central Research Science Laboratory, the Core Laboratory Facilities, the Affymetrix Core Facility at American University of Beirut, and Ms. Nadine Makhoul for their help and expert input. The authors acknowledge the financial support of the L’Oréal-UNESCO For Women in Science 2014 Levant and Egypt and the American University of Beirut Medical Practice Plan.
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Nassar, F.J., El Eit, R., Nasr, R. (2016). An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells. In: Li, S., Zhang, H. (eds) Chronic Myeloid Leukemia. Methods in Molecular Biology, vol 1465. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4011-0_18
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DOI: https://doi.org/10.1007/978-1-4939-4011-0_18
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Publisher Name: Humana Press, New York, NY
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