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Midline (Central) Fluid Percussion Model of Traumatic Brain Injury

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1462)

Abstract

Research models of traumatic brain injury (TBI) hold significant validity towards the human condition, with each model replicating a subset of clinical features and symptoms. After 30 years of characterization and implementation, fluid percussion injury (FPI) is firmly recognized as a clinically relevant model of TBI, encompassing concussion through severe injury. The midline variation of FPI may best represent mild and diffuse clinical brain injury, because of the acute behavioral deficits, the late onset of subtle behavioral morbidities, and the absence of gross histopathology. This chapter outlines the procedures for midline (diffuse) FPI in adult male rats and mice. With these procedures, it becomes possible to generate brain-injured laboratory animals for studies of injury-induced pathophysiology and behavioral deficits, for which rational therapeutic interventions can be implemented.

Key words

Traumatic brain injury (TBI) Concussion Diffuse Fluid percussion Trauma Rodent Rat Mouse Experimental model Righting reflex Fencing response Postoperative care 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.BARROW Neurological Institute at Phoenix Children’s HospitalPhoenixUSA
  2. 2.Department of Child HealthUniversity of Arizona College of Medicine - PhoenixPhoenixUSA
  3. 3.Phoenix Veteran Affairs Healthcare SystemPhoenixUSA

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