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Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo

  • Wenxiao Wu
  • Laizhong Chen
  • Jing Li
  • Lupei Du
  • Minyong LiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one of the most popular bioluminescence system, the firefly luciferin–luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.

Key words

Aminopeptidase N Bioluminescence Caged luciferin Living animal imaging 

Notes

Acknowledgement

This work was supported by grants from the Program of New Century Excellent Talents in University (No. NCET-11-0306), the Shandong Natural Science Foundation (No. JQ201019), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13028), the Major Project of Science and Technology of Shandong Province (No. 2015ZDJS04001), the Shandong Key Research & Development Project (No. 2015GSF118166) and the Independent Innovation Foundation of Shandong University, IIFSDU (No. 2010JQ005 and 2014JC008). The first two authors contributed to this article equally.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Wenxiao Wu
    • 1
  • Laizhong Chen
    • 1
  • Jing Li
    • 1
  • Lupei Du
    • 1
  • Minyong Li
    • 1
    Email author
  1. 1.Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of PharmacyShandong UniversityJinanChina

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