Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo
Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one of the most popular bioluminescence system, the firefly luciferin–luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.
Key wordsAminopeptidase N Bioluminescence Caged luciferin Living animal imaging
This work was supported by grants from the Program of New Century Excellent Talents in University (No. NCET-11-0306), the Shandong Natural Science Foundation (No. JQ201019), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13028), the Major Project of Science and Technology of Shandong Province (No. 2015ZDJS04001), the Shandong Key Research & Development Project (No. 2015GSF118166) and the Independent Innovation Foundation of Shandong University, IIFSDU (No. 2010JQ005 and 2014JC008). The first two authors contributed to this article equally.
- 6.Noble F, Banisadr G, Jardinaud F, Popovici T, Lai-Kuen R, Chen H, Bischoff L, Parsadaniantz SM, Fournie-Zaluski MC, Roques BP (2001) First discrete autoradiographic distribution of aminopeptidase N in various structures of rat brain and spinal cord using the selective iodinated inhibitor [125I]RB 129. Neuroscience 105:479–488CrossRefPubMedGoogle Scholar
- 7.Vlahovic P, Stefanovic V (1998) Kidney ectopeptidases. Structure, functions and clinical significance. Pathol Biol (Paris) 46:779–786Google Scholar