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Quantum Yield Determination Based on Photon Number Measurement, Protocols for Firefly Bioluminescence Reactions

  • Kazuki NiwaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

Quantum yield (QY), which is defined as the probability of photon production by a single bio/chemiluminescence reaction, is an important factor to characterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine QY according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are introduced.

Key words

Firefly luciferase Quantum yield Bioluminescence Luminometer National radiometry standard 

Notes

Acknowledgement

K.N. would like to thank Y. Ichino for helpful discussions about absolute calibrations and Y. Ohmiya for his discussions about bioluminescence reactions. This work was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Quantum Optical Measurement Group, National Metrology Institute of JapanNational Institute of Advanced Industrial Science and TechnologyTsukubaJapan

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