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Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepods

  • Yasuhiro Takenaka
  • Kazuho Ikeo
  • Yasushi ShigeriEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

Secreted luciferases isolated from copepod crustaceans are frequently used for nondisruptive reporter-gene assays, such as the continuous, automated and/or high-throughput monitoring of gene expression in living cells. All known copepod luciferases share highly conserved amino acid residues in two similar, repeated domains in the sequence. The similarity in the domains are ideal nature for designing PCR primers to amplify cDNA fragments of unidentified copepod luciferases from bioluminescent copepod crustaceans. Here, we introduce how to establish a cDNA encoding novel copepod luciferases from a copepod specimen by PCR with degenerated primers.

Key words

Copepod GLuc Luciferase MLuc MpLuc Plankton 

Notes

Acknowledgement

We are grateful to Prof. Gary Wayman (Washington State University, Pullman) for his careful reading of our manuscript. This work was supported by an AIST research grant and the NIG Cooperative Research Program (Y. Shigeri and K. Ikeo, 2014-A63, 2015-A47).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yasuhiro Takenaka
    • 1
  • Kazuho Ikeo
    • 2
  • Yasushi Shigeri
    • 3
    Email author
  1. 1.Department of Diabetes and EndocrinologySaitama Medical UniversityIruma-gunJapan
  2. 2.Center for Information BiologyNational Institute of GeneticsMishimaJapan
  3. 3.Health Research InstituteNational Institute of Advanced Industrial Science and Technology (AIST)IkedaJapan

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