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Photon Counting System for High-Sensitivity Detection of Bioluminescence at Optical Fiber End

  • Masataka IinumaEmail author
  • Yutaka Kadoya
  • Akio Kuroda
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

The technique of photon counting is widely used for various fields and also applicable to a high-sensitivity detection of luminescence. Thanks to recent development of single photon detectors with avalanche photodiodes (APDs), the photon counting system with an optical fiber has become powerful for a detection of bioluminescence at an optical fiber end, because it allows us to fully use the merits of compactness, simple operation, highly quantum efficiency of the APD detectors. This optical fiber-based system also has a possibility of improving the sensitivity to a local detection of Adenosine triphosphate (ATP) by high-sensitivity detection of the bioluminescence. In this chapter, we are introducing a basic concept of the optical fiber-based system and explaining how to construct and use this system.

Key words

Photon counting Optical fiber Single photon detector Avalanche photodiode (APD) High sensitivity Adenosine triphosphate Luciferase Bioluminescence 

Notes

Acknowledgement

This work has been partially supported by the International Project Center for Integration Research on Quantum, Information, and Life Sciences of Hiroshima University and the Grant-in-Aid for Scientific Research (C) (19560046) of Japanese Society for the Promotion of Science, JSPS.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Graduate school of Advanced Sciences of MatterHiroshima UniversityHigashi-HiroshimaJapan

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