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Luminescence Imaging: (a) Multicolor Visualization of Ca2+ Dynamics in Different Cellular Compartments and (b) Video-Rate Tumor Detection in a Freely Moving Mouse

  • Kenta Saito
  • Masahiro Nakano
  • Takeharu NagaiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

Luminescence exerts an ideal optical readout for imaging living subjects including no external light source, whereas the dim luminescence and poor color pallet should be addressed for the better utilities. To address the demerits and to prevail the advantages, we developed a bright luminescent protein, named yellow Nano-lantern, exhibiting about 10–20 times brighter than wild-type RLuc. In this chapter, we demonstrate two luminescence-based protocols in detail: i.e., (a) multicolor visualization of Ca2+ dynamics in different cellular compartments in a single cell using Ca2+ indicators based on cyan- and orange-Nano-lanterns and (b) video-rate tumor detection in a freely moving mouse using yellow Nano-lantern.

Key words

Bioluminescence Calcium dynamics Tumor imaging Renilla luciferase Bioluminescence resonance energy transfer (BRET) 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.The Center for Brain Integration ResearchTokyo Medical and Dental UniversityTokyoJapan
  2. 2.The Institute of Scientific and Industrial ResearchOsaka UniversityIbarakiJapan

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