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A Multichannel Bioluminescence Determination Platform for Bioassays

  • Sung-Bae KimEmail author
  • Ryuichi Naganawa
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

The present protocol introduces a multichannel bioluminescence determination platform allowing a high sample throughput determination of weak bioluminescence with reduced standard deviations. The platform is designed to carry a multichannel conveyer, an optical filter, and a mirror cap. The platform enables us to near-simultaneously determine ligands in multiple samples without the replacement of the sample tubes. Furthermore, the optical filters beneath the multichannel conveyer are designed to easily discriminate colors during assays. This optical system provides excellent time- and labor-efficiency to users during bioassays.

Key words

Cortisol Steroid hormone Bioluminescence Assay device Single-chain probe 

Notes

Acknowledgements

This work was supported by grants from Japan Society for the Promotion of Science (JSPS), grants number 26288088, 16K14051, and 15KK0029.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Research Institute for Environmental Management TechnologyNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan

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