A Multichannel Bioluminescence Determination Platform for Bioassays
The present protocol introduces a multichannel bioluminescence determination platform allowing a high sample throughput determination of weak bioluminescence with reduced standard deviations. The platform is designed to carry a multichannel conveyer, an optical filter, and a mirror cap. The platform enables us to near-simultaneously determine ligands in multiple samples without the replacement of the sample tubes. Furthermore, the optical filters beneath the multichannel conveyer are designed to easily discriminate colors during assays. This optical system provides excellent time- and labor-efficiency to users during bioassays.
Key wordsCortisol Steroid hormone Bioluminescence Assay device Single-chain probe
This work was supported by grants from Japan Society for the Promotion of Science (JSPS), grants number 26288088, 16K14051, and 15KK0029.