Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays

  • G. V. M. Gabriel
  • V. R. VivianiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)


We describe a simple light off bioluminescent biosensor for general environmental toxicity assays based on immobilized bioluminescent bacteria engineered with beetle luciferases.

Key words

Bioluminescent biosensor Toxicity biosensor pH-sensitive firefly luciferase 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Laboratory of Biochemistry and Biotechnology of Bioluminescence, Department of Physics, Chemistry and MathematicsFederal University of São Carlos (UFSCar)SorocabaBrazil
  2. 2.Graduate School of Biotechnology and Environmental MonitoringFederal University of São Carlos (UFSCar)SorocabaBrazil
  3. 3.Graduate School of Evolutive Genetics and Molecular BiologyFederal University of São Carlos (UFSCar)São CarlosBrazil

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