Live Cell Bioluminescence Imaging in Temporal Reaction of G Protein-Coupled Receptor for High-Throughput Screening and Analysis
G protein-coupled receptors (GPCRs) are notable targets of basic therapeutics. Many screening methods have been established to identify novel agents for GPCR signaling in a high-throughput manner. However, information related to the temporal reaction of GPCR with specific ligands remains poor. We recently developed a bioluminescence method for the quantitative detection of the interaction between GPCR and β-arrestin using split luciferase complementation. To monitor time-course variation of the interactions, a new imaging system contributes to the accurate evaluation of drugs for GPCRs in a high-throughput manner.
Key wordsG protein-coupled receptor Bioluminescence Complementary technology Protein–protein interaction High-throughput screening EM-CCD camera
We thank Molecular Devices Corp. for active support related to the technical aspects of imaging. This work was supported by the Japan Society for the Promotion of Science (JSPS) and MEXT, Japan.
- 16.Kafi AKM, Hattori M, Misawa N, Ozawa T (2011) Dual-color bioluminescence analysis for quantitatively monitoring G-protein-coupled receptor and β-arrestin interactions. Pharmaceuticals 4:457–469Google Scholar