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Fabrication of Molecular Strain Probes for Illuminating Protein–Protein Interactions

  • Sung-Bae KimEmail author
  • Rika Fujii
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

A unique bioluminescent imaging probe is introduced for illuminating molecular tension appended by protein–protein interactions (PPIs) of interest. A full-length luciferase is sandwiched between two proteins of interest via minimal flexible linkers. The ligand-activated PPIs append intramolecular tension to the sandwiched luciferase, boosting or dropping the enzymatic activity in a quantitative manner. This method guides construction of a new lineage of bioassays for ligand-activated PPIs.

Key words

Luciferase Bioluminescence Molecular tension Protein–protein interactions 

Notes

Acknowledgements

This work was supported by grants from Japan Society for the Promotion of Science (JSPS), grant numbers 26288088, 16K14051, and 15KK0029.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan

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