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Label-Free Cell Phenotypic Identification of d-Luciferin as an Agonist for GPR35

  • Heidi Hu
  • Huayun Deng
  • Ye FangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Abstract

d-Luciferin (also known as beetle or firefly luciferin) is one of the most widely used bioluminescent reporters for monitoring in vitro or in vivo luciferase activity. The identification of several natural phenols and thieno[3,2-b]thiophene-2-carboxylic acid derivatives as agonists for GPR35, an orphan G protein-coupled receptor, had motivated us to examine the pharmacological activity of d-Luciferin, given that it also contains phenol and carboxylic acid moieties. Here, we describe label-free cell phenotypic assays that ascertain d-Luciferin as a partial agonist for GPR35. The agonistic activity of d-Luciferin at the GPR35 shall evoke careful interpretation of biological data when d-Luciferin or its analogues are used as probes.

Key words

Bioluminescence Dynamic mass redistribution G protein-coupled receptor GPR35 d-Luciferin Resonant waveguide grating biosensor 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Medical Laboratory Science, Jefferson College of Health Science/Carilion Roanoke Memorial HospitalQuest Diagnostic Lab, Carilion Clinic Health SystemRoanokeUSA
  2. 2.Biochemical Technologies, Science and Technology DivisionCorning IncorporatedCorningUSA

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