Abstract
Mitochondrial function is a key component of skeletal muscle health, and its dysfunction has been associated with a wide variety of diseases. Microplate-based respirometry measures aerobic respiration of live cells through extracellular changes in oxygen concentration. Here, we describe a methodology to measure aerobic respiration of intact murine skeletal muscle tissue. The tissues are not cultured, permeabilized, or enzymatically dissociated to single fibers, so there is minimal experimental manipulation affecting the samples prior to acquiring measurements.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kelley DE, He J, Menshikova EV, Ritov VB (2002) Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes. Diabetes 51(10):2944–2950
Lindroos MM, Majamaa K, Tura A, Mari A, Kalliokoski KK, Taittonen MT, Iozzo P, Nuutila P (2009) m.3243A > G mutation in mitochondrial DNA leads to decreased insulin sensitivity in skeletal muscle and to progressive beta-cell dysfunction. Diabetes 58(3):543–549. doi:10.2337/db08-0981
Taylor DJ, Kemp GJ, Thompson CH, Radda GK (1997) Ageing: effects on oxidative function of skeletal muscle in vivo. Mol Cell Biochem 174(1-2):321–324
Conley KE, Jubrias SA, Esselman PC (2000) Oxidative capacity and ageing in human muscle. J Physiol 526(Pt 1):203–210
Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS (2005) Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci U S A 102(15):5618–5623. doi:10.1073/pnas.0501559102
Boncompagni S, Moussa CE, Levy E, Pezone MJ, Lopez JR, Protasi F, Shtifman A (2012) Mitochondrial dysfunction in skeletal muscle of amyloid precursor protein-overexpressing mice. J Biol Chem 287(24):20534–20544. doi:10.1074/jbc.M112.359588
Lalani SR, Vladutiu GD, Plunkett K, Lotze TE, Adesina AM, Scaglia F (2005) Isolated mitochondrial myopathy associated with muscle coenzyme Q10 deficiency. Arch Neurol 62(2):317–320. doi:10.1001/archneur.62.2.317
Holt IJ, Harding AE, Morgan-Hughes JA (1988) Deletions of muscle mitochondrial DNA in patients with mitochondrial myopathies. Nature 331(6158):717–719. doi:10.1038/331717a0
Nakamura S, Sato T, Hirawake H, Kobayashi R, Fukuda Y, Kawamura J, Ujike H, Horai S (1990) In situ hybridization of muscle mitochondrial mRNA in mitochondrial myopathies. Acta Neuropathol 81(1):1–6
Watanabe M, Houten SM, Mataki C, Christoffolete MA, Kim BW, Sato H, Messaddeq N, Harney JW, Ezaki O, Kodama T, Schoonjans K, Bianco AC, Auwerx J (2006) Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation. Nature 439(7075):484–489. doi:10.1038/nature04330
Wu M, Neilson A, Swift AL, Moran R, Tamagnine J, Parslow D, Armistead S, Lemire K, Orrell J, Teich J, Chomicz S, Ferrick DA (2007) Multiparameter metabolic analysis reveals a close link between attenuated mitochondrial bioenergetic function and enhanced glycolysis dependency in human tumor cells. Am J Physiol Cell Physiol 292(1):C125–C136. doi:10.1152/ajpcell.00247.2006
Piper HM, Sezer O, Schleyer M, Schwartz P, Hutter JF, Spieckermann PG (1985) Development of ischemia-induced damage in defined mitochondrial subpopulations. J Mol Cell Cardiol 17(9):885–896
Milner DJ, Mavroidis M, Weisleder N, Capetanaki Y (2000) Desmin cytoskeleton linked to muscle mitochondrial distribution and respiratory function. J Cell Biol 150(6):1283–1298
Kay L, Nicolay K, Wieringa B, Saks V, Wallimann T (2000) Direct evidence for the control of mitochondrial respiration by mitochondrial creatine kinase in oxidative muscle cells in situ. J Biol Chem 275(10):6937–6944
Saks VA, Belikova YO, Kuznetsov AV (1991) In vivo regulation of mitochondrial respiration in cardiomyocytes: specific restrictions for intracellular diffusion of ADP. Biochim Biophys Acta 1074(2):302–311
Veksler VI, Kuznetsov AV, Sharov VG, Kapelko VI, Saks VA (1987) Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers. Biochim Biophys Acta 892(2):191–196
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat Protoc 3(6):965–976. doi:10.1038/nprot.2008.61
Wenz T, Rossi SG, Rotundo RL, Spiegelman BM, Moraes CT (2009) Increased muscle PGC-1alpha expression protects from sarcopenia and metabolic disease during aging. Proc Natl Acad Sci U S A 106(48):20405–20410. doi:10.1073/pnas.0911570106
McCarroll SA, Murphy CT, Zou S, Pletcher SD, Chin CS, Jan YN, Kenyon C, Bargmann CI, Li H (2004) Comparing genomic expression patterns across species identifies shared transcriptional profile in aging. Nat Genet 36(2):197–204. doi:10.1038/ng1291
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Shintaku, J., Guttridge, D.C. (2016). Analysis of Aerobic Respiration in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry. In: Kyba, M. (eds) Skeletal Muscle Regeneration in the Mouse. Methods in Molecular Biology, vol 1460. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3810-0_23
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3810-0_23
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-3808-7
Online ISBN: 978-1-4939-3810-0
eBook Packages: Springer Protocols