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Role and Characterization of Synuclein-γ Unconventional Protein Secretion in Cancer Cells

  • Caiyun Liu
  • Like Qu
  • Chengchao Shou
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1459)

Abstract

Synuclein-γ (SNCG), the third member of synuclein family, is implicated in both neurodegenerative diseases and cancer. Overexpression of SNCG in cancer cells is linked to tumor progression and chemoresistance. Without any known signal sequence required for conventional protein secretion, SNCG is elevated in the serum of cancer patients and the medium of cultured cancer cells. SNCG actively secretes from cancer cells and extracellular SNCG promotes malignant phenotypes of cancer cells. Here, we describe methods for the characterization of SNCG as an unconventional secretion protein from cancer cells and investigation of the effect of extracellular SNCG on the phenotypes of cancer cells.

Key words

Synuclein-γ Unconventional secretion Cancer cell Migration Invasion 

Notes

Acknowledgement

This work was supported by the National Natural Science Foundation of China (No. 81272410).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital & InstituteBeijingChina
  2. 2.Department of Biochemistry & Molecular BiologyPeking University Cancer Hospital & InstituteBeijingChina

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