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Modeling Breast Tumor Development with a Humanized Mouse Model

  • Lisa M. Arendt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1458)

Abstract

The tumor microenvironment plays a critical role in breast cancer growth and progression to metastasis. Here, we describe a method to examine stromal–epithelial interactions during tumor formation and progression utilizing human-derived mammary epithelial cells and breast stromal cells. This method outlines the isolation of each cell type from reduction mammoplasty tissue, the culture and genetic modification of both epithelial and stromal cells using lentiviral technology, and the method of humanizing and implantation of transformed epithelial cells into the cleared mammary fat pads of immunocompromised mice. This model system may be a useful tool to dissect signaling interactions that contribute to invasive tumor behavior and therapeutic resistance.

Key words

Human-in-Mouse model Human mammary epithelial cells Breast cancer Stroma Mammary gland Stromal–epithelial interactions 

Notes

Acknowledgements

The author would like to thank Victoria Thompson for helpful discussions. This work is supported by the Susan G. Komen Foundation.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Comparative Biosciences, School of Veterinary MedicineUniversity of Wisconsin-MadisonMadisonUSA

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