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Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1458)

Abstract

Patient-derived xenograft (PDX) models provide an excellent platform to understand cancer initiation and development in vivo. In the context of brain tumor initiating cells (BTICs), PDX models allow for characterization of tumor formation, growth, and recurrence, in a clinically relevant in vivo system. Here, we detail procedures to harvest, culture, characterize, and orthotopically inject human BTICs derived from patient samples.

Key words

Patient-derived xenograft (PDX) Brain tumor-initiating cell (BTIC) Flow cytometry In vitro self-renewal assay In vitro proliferation assay Lentivirus Intracranial injections Immunohistochemistry NOD-SCID 
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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.McMaster Stem Cell and Cancer Research InstituteMcMaster UniversityHamiltonCanada
  2. 2.Department of Biochemistry and Biomedical Sciences, Faculty of Health SciencesMcMaster UniversityHamiltonCanada
  3. 3.Department of Surgery, Faculty of Health SciencesMcMaster UniversityHamiltonCanada

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